Mouse megakaryocytes secrete acetylcholinesterase
نویسندگان
چکیده
منابع مشابه
Immature human megakaryocytes produce nuclear-associated acetylcholinesterase.
Acetylcholinesterase (AChE) is expressed in murine megakaryocytes (MK), where its antisense inhibition suppresses differentiation, yet was never detected in human MK. Here, we report that AChE is produced in normal human bone marrow MK and in cell lines derived thereof. Reverse transcriptase-polymerase chain reaction (RT-PCR) amplification showed two ACHEmRNA forms in human megakaryoblastic DAM...
متن کاملImmature Megakaryocytes in the Mouse: Morphology and quantitation by acetylcholinesterase staining.
Three types of immature megakaryocytes, detected by their morphological properties, have been characterized in bone marrow of normal C57BL/6 mice. Morphological classification of these cells was carried out by determining (1) presence and relative amount of acetylcholinesterase, (2) cell size, (3) nuclear/cytoplasm ratio, and (4) nuclear shape. The immature megakaryocytes were classified as: (A...
متن کاملSurvivin localization during endomitosis of high ploidy mouse megakaryocytes.
During polyploidization the megakaryocyte (MK) undergoes endomitosis, which involves skipping late anaphase and cytokinesis.1,2 As a regulator of anaphase and cytokinesis, our laboratory implicated survivin in this process due to the absence of survivin protein in endomitotic mouse MKs,3 which are typically of high ploidy. Gurbuxani et al4 also noted survivin’s down-regulation at the mRNA level...
متن کاملImmature Megakaryocytes in the Mouse : Physical Characteristics , Cell Cycle
The heterogeneity among immature megakaryocytes has been examined by physical properties. cell cycle status. and responsiveness to thrombopoietic stimulatory factor. Three types of immature megakaryocytes exist that can be recognized by acetylcholinesterase staining. nuclear shape. high nucleus/cytoplasm ratio, and small size (818 ) with respect to mature megakaryocytes (>18 ii). These three ac...
متن کاملChronic acetylcholinesterase overexpression induces multilevelled aberrations in mouse neuromuscular physiology.
Chronic overexpression of the acetylcholine-hydrolysing enzyme acetylcholinesterase (AChE) is a notable consequence of exposure to anticholinesterase drugs or poisons. However, the physiological consequences for the resultant neuromuscular disfunction have not yet been carefully analysed. Here we report detailed dissection of the different components of neuromuscular function in transgenic mice...
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ژورنال
عنوان ژورنال: Blood
سال: 1981
ISSN: 0006-4971,1528-0020
DOI: 10.1182/blood.v58.6.1100.1100